Tracy GUI

Sign in with your Bioagens account to assemble Sanger chromatograms.

/ Sanger Chromatogram Assembly

Upload a single-read Sanger trace (.ab1), get the consensus sequence with per-base Phred quality, and optionally call variants against a reference.

Chromatogram trace (.ab1)

Drag a .ab1 file here, or click to browse

If provided, Tracy will decompose the trace against this reference and call variants (VCF download).

Results will appear here.

Running Tracy...

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Length (bp)

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Mean Phred Q

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Bases < Q20

Per-base Phred quality

<Q20 Q20-40 >Q40

Consensus sequence (FASTA)

Variants vs. reference